Heat-shock protein 83 of Leishmania mexicana amazonensis is an abundant cytoplasmic protein with a tandemly repeated genomic arrangement

Contents

The 83-kDa heat-shock protein HSP83 is a extremely plentiful protein in Leishmania amastigotes and promastigotes uncovered to elevated temperature. Antibodies towards this protein have been obtained by immunization with an artificial peptide derived from a conserved area.

 

These antibodies acknowledged each the denatured and the native type of the molecule and have been used for immunofluorescence evaluation. These experiments, along with evaluation by cell fractionation, present that HSP83 is distributed within the cytoplasm of Leishmania parasites.

 

The gene for HSP83 in Leishmania mexicana amazonensis has been cloned from a genomic library, and molecular characterization exhibits it’s current in a number of copies of 4-kb repeats organized in tandem.

  • Kinetoplastid parasites of the Leishmania genus trigger a number of types of leishmaniasis. Leishmania species pathogenic to human are separated into two subgenera, Leishmania (Leishmania) and L. (Viannia).

  • Species from the Viannia subgenus trigger predominantly cutaneous leishmaniasis in Central and South America, often resulting in extra extreme medical shows. Though the genomes of a number of species of Leishmania have been sequenced thus far, just one belongs to this slightly totally different subgenus.

 

  • Right here we discover the distinctive options of the Viannia subgenus by sequencing and analyzing the genome of L. (Viannia) panamensis. Towards a background of conservation in gene content material and synteny, we discovered key variations on the genomic stage that will clarify the prevalence of molecular processes involving nucleic acid manipulation and differential modification of floor glycoconjugates.

Hypersensitive ELISA Luminous substrate

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Low background ECL luminous Detection Kit Instruction

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High background ECL luminous Detection Kit Instruction

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WHATMAN(TM) MEMBRANE MARKING PEN 10/PK - EACH

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Membrane Cofactor Protein (MCP) Magnetic Luminex Assay Kit

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Epithelial Membrane Protein 3 (EMP3) Magnetic Luminex Assay Kit

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TSPAN33, CT (TSPAN33, PEN, Tetraspanin-33, Penumbra, Proerythroblast new membrane)

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TSPAN33, CT (TSPAN33, PEN, Tetraspanin-33, Penumbra, Proerythroblast new membrane)

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Membrane Protein, Palmitoylated 6 (MPP6) Magnetic Luminex Assay Kit

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Erythrocyte Membrane Protein Band 4.2 (EPB42) Magnetic Luminex Assay Kit

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Glycosylated Lysosomal Membrane Protein (GLMP) Magnetic Luminex Assay Kit

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TSPAN33, CT (TSPAN33, PEN, Tetraspanin-33, Penumbra, Proerythroblast new membrane) (AP)

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TSPAN33, CT (TSPAN33, PEN, Tetraspanin-33, Penumbra, Proerythroblast new membrane) (AP)

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TSPAN33, CT (TSPAN33, PEN, Tetraspanin-33, Penumbra, Proerythroblast new membrane) (PE)

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TSPAN33, CT (TSPAN33, PEN, Tetraspanin-33, Penumbra, Proerythroblast new membrane) (PE)

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Glycoprotein 2, Zymogen Granule Membrane (GP2) Magnetic Luminex Assay Kit

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TSPAN33, CT (TSPAN33, PEN, Tetraspanin-33, Penumbra, Proerythroblast new membrane) (FITC)

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Lysosomal Associated Membrane Protein 1 (LAMP1) Magnetic Luminex Assay Kit

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ATPase, Ca++ Transporting, Plasma Membrane 2 (ATP2B2) Magnetic Luminex Assay Kit

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TSPAN33, CT (TSPAN33, PEN, Tetraspanin-33, Penumbra, Proerythroblast new membrane) (Biotin)

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TSPAN33, CT (TSPAN33, PEN, Tetraspanin-33, Penumbra, Proerythroblast new membrane) (Biotin)

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Membrane Spanning 4 Domains Subfamily A, Member 1 (CD20) Magnetic Luminex Assay Kit

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Adipocyte Plasma Membrane Associated Protein (APMAP) Magnetic Luminex Assay Kit

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Translocation Associated Membrane Protein 1 (TRAM1) Magnetic Luminex Assay Kit

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BMP And Activin Membrane Bound Inhibitor Homolog (BAMBI) Magnetic Luminex Assay Kit

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TSPAN33, CT (TSPAN33, PEN, Tetraspanin-33, Penumbra, Proerythroblast new membrane) (MaxLight 405)

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TSPAN33, CT (TSPAN33, PEN, Tetraspanin-33, Penumbra, Proerythroblast new membrane) (MaxLight 490)

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TSPAN33, CT (TSPAN33, PEN, Tetraspanin-33, Penumbra, Proerythroblast new membrane) (MaxLight 490)

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TSPAN33, CT (TSPAN33, PEN, Tetraspanin-33, Penumbra, Proerythroblast new membrane) (MaxLight 550)

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TSPAN33, CT (TSPAN33, PEN, Tetraspanin-33, Penumbra, Proerythroblast new membrane) (MaxLight 550)

MBS6359613-5x01mL 5x0.1mL
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TSPAN33, CT (TSPAN33, PEN, Tetraspanin-33, Penumbra, Proerythroblast new membrane) (MaxLight 650)

MBS6359614-01mL 0.1mL
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TSPAN33, CT (TSPAN33, PEN, Tetraspanin-33, Penumbra, Proerythroblast new membrane) (MaxLight 650)

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EUR 4250

TSPAN33, CT (TSPAN33, PEN, Tetraspanin-33, Penumbra, Proerythroblast new membrane) (MaxLight 750)

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TSPAN33, CT (TSPAN33, PEN, Tetraspanin-33, Penumbra, Proerythroblast new membrane) (Azide free) (HRP)

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EUR 650

Recombinant Photorhabdus luminescens subsp. laumondii Major outer membrane lipoprotein (lpp)

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Recombinant Photorhabdus luminescens subsp. laumondii UPF0208 membrane protein plu3094 (plu3094)

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Recombinant Photorhabdus luminescens subsp. laumondii UPF0208 membrane protein plu3094 (plu3094)

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Recombinant Photorhabdus luminescens subsp. laumondii UPF0208 membrane protein plu3094 (plu3094)

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Recombinant Photorhabdus luminescens subsp. laumondii UPF0259 membrane protein plu2479 (plu2479)

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Recombinant Photorhabdus luminescens subsp. laumondii UPF0259 membrane protein plu2479 (plu2479)

MBS7039179-01mg 0.1mg
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Recombinant Photorhabdus luminescens subsp. laumondii UPF0259 membrane protein plu2479 (plu2479)

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Recombinant Photorhabdus luminescens subsp. laumondii UPF0266 membrane protein plu2700 (plu2700)

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Recombinant Photorhabdus luminescens subsp. laumondii UPF0266 membrane protein plu2700 (plu2700)

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EUR 2390

Recombinant Photorhabdus luminescens subsp. laumondii UPF0266 membrane protein plu2700 (plu2700)

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EUR 10715

Recombinant Photorhabdus luminescens subsp. laumondii UPF0283 membrane protein plu2581 (plu2581)

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Recombinant Photorhabdus luminescens subsp. laumondii UPF0283 membrane protein plu2581 (plu2581)

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Recombinant Photorhabdus luminescens subsp. laumondii UPF0283 membrane protein plu2581 (plu2581)

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Recombinant Photorhabdus luminescens subsp. laumondii UPF0299 membrane protein plu1549 (plu1549)

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Recombinant Photorhabdus luminescens subsp. laumondii UPF0299 membrane protein plu1549 (plu1549)

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EUR 2385

Recombinant Photorhabdus luminescens subsp. laumondii UPF0299 membrane protein plu1549 (plu1549)

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EUR 10680

Recombinant Photorhabdus luminescens subsp. laumondii UPF0756 membrane protein plu2726 (plu2726)

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EUR 1425

Recombinant Photorhabdus luminescens subsp. laumondii UPF0756 membrane protein plu2726 (plu2726)

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EUR 2390

Recombinant Photorhabdus luminescens subsp. laumondii UPF0756 membrane protein plu2726 (plu2726)

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EUR 10710

Recombinant Photorhabdus luminescens subsp. laumondii UPF0208 membrane protein plu3094 (plu3094), partial

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Recombinant Photorhabdus luminescens subsp. laumondii UPF0299 membrane protein plu1549 (plu1549), partial

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  • These variations could partially clarify some phenotypic traits of the Viannia parasites, together with their elevated adaptive capability and enhanced metastatic potential.

Genomic construction and RNA polymerase exercise in Leishmania virus.

  • Over the last decade miltefosine (MIL) has been used as first-line remedy for visceral leishmaniasis in endemic areas with antimonial resistance, however a decline in medical effectiveness is now being reported.

 

  • Whereas solely two MIL-resistant Leishmania infantum strains from HIV co-infected sufferers have been documented, phenotypic MIL-resistance for L. donovani has not but been recognized within the laboratory. Therefore, a greater understanding of the components contributing to elevated MIL-treatment failure is critical. Given the paucity of outlined MIL-resistant L. donovani medical isolates, this research used an experimental amastigote-selected MIL-resistant L. infantum isolate (LEM3323). In-depth exploration of the MIL-resistant phenotype was carried out by coupling genomic with phenotypic knowledge to realize perception into gene perform and the mutant phenotype.

 

  • A naturally MIL-resistant L. infantum medical isolate (LEM5159) was included to check each datasets. Phenotypically, resistance was evaluated by figuring out intracellular amastigote susceptibility in vitro and precise MIL-uptake. Genomic evaluation offered supportive proof that the resistance choice mannequin on intracellular amastigotes is usually a good proxy for the in vivo discipline state of affairs since each resistant strains confirmed mutations in the identical inward transporter system chargeable for the acquired MIL-resistant phenotype.

 

  • Consistent with earlier literature findings in promastigotes, our knowledge affirm a faulty import equipment by means of inactivation of the LiMT/LiRos3 protein advanced as the primary mechanism for MIL-resistance additionally in intracellular amastigotes. Complete genome sequencing evaluation of LEM3323 revealed a 2 base pair deletion within the LiMT gene that led to the formation an early cease codon and a truncation of the LiMT protein. Apparently, LEM5159 revealed mutations in each the LiMT and LiRos3 genes, leading to an aberrant expression of the LiMT protein.
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Human Interleukin 17C,IL-17C ELISA KIT
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Human Interleukin 17C(IL-17C)ELISA Kit
QY-E04320 96T
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Human Interleukin 17C, IL-17C ELISA Kit
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Human Interleukin 17C, IL-17C ELISA Kit
MBS163764-48StripWells 48-Strip-Wells
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Human Interleukin 17C, IL-17C ELISA Kit
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Human Interleukin 17C, IL-17C ELISA Kit
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Human Interleukin 17C (IL-17C) ELISA Kit
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Human Interleukin 17C (IL-17C) ELISA Kit
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Human Interleukin 17C (IL-17C) ELISA Kit
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EUR 3420
Human Interleukin 17C (IL-17C) ELISA Kit
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EUR 765
Human Interleukin 17C,IL-17C ELISA Kit
201-12-2161 96 tests
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Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.
Human Interleukin 17C, IL-17C GENLISA ELISA
KBH2160 1 x 96 wells
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Interleukin 17C (IL-17C)
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EUR 850
Interleukin 17C (IL-17C)
MBS600337-5x01mg 5x0.1mg
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Anti-Human IL-17C Antibody
101-M487 100 µg
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Description: The IL-17 family is comprised of at least six proinflammatory cytokines that share a conserved cysteine-knot structure but diverge at the N-terminus. IL-17 family members are glycoproteins secreted as dimers that induce local cytokine production and recruit granulocytes to sites of inflammation. IL-17 is induced by IL-15 and IL-23, mainly in activated CD4+ T cells distinct from Th1 or Th2 cells. IL-17F is the most homologous to IL-17, but is induced only by IL-23 in activated monocytes. IL-17B binds the IL-17B receptor, but not the IL-17 receptor; it is most homologous with IL-17D, which is expressed by resting CD4+ T cells and CD19+ B cells. IL-17E is mainly produced by Th2 cells and recruits eosinophils to lung tissue. IL-17C has a very restricted expression pattern but has been detected in adult prostate and fetal kidney libraries.
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Description: Human Interleukin-17c (IL-17c) ELISA Kit
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Human Interleukin-17c (IL-17c) ELISA Kit
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Anti-IL-17C Antibody
A00164 100ul
EUR 380
Description: Boster Bio Anti-IL-17C Antibody catalog # A00164. Tested in ELISA, IHC applications. This antibody reacts with Human.
Anti-IL-17C antibody
STJ98799 200 µl
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Description: Rabbit polyclonal to IL-17C.
Anti-IL-17C Antibody
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Anti-IL-17C Antibody
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Anti-IL-17C Antibody
MBS8291657-02mL 0.2mL
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Anti-IL-17C Antibody
MBS8291657-5x02mL 5x0.2mL
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Interleukin 17C (IL-17C) (Carboxyfluorescein)
MBS600196-100Tests 100Tests
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Interleukin 17C (IL-17C) (Carboxyfluorescein)
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Anti-Human IL-17C Azide Free
MBS335832-02mg 0.2mg
EUR 290
Anti-Human IL-17C Azide Free
MBS335832-05mg 0.5mg
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Anti-Human IL-17C Azide Free
MBS335832-5x05mg 5x0.5mg
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Anti-Human IL-17C Azide Free
MBS335843-02mg 0.2mg
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Anti-Human IL-17C Azide Free
MBS335843-05mg 0.5mg
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Anti-Human IL-17C Azide Free
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Mouse Monoclonal anti-human IL-17C
hAP-0375 100ug
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Human IL-17c, Fc Tag
E24PHA370 50 μg
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Human IL-17c, His Tag
E24PHA151 50 μg
EUR 262
Human IL-17C ELISA Kit
E16HE0251 96T
EUR 833.33
IL-17C (Human) ELISA Kit
EKA51955-5x96T 5x96T Ask for price
IL-17C (Human) ELISA Kit
EKA51955-96T 96T
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Human IL-17C ELISA Kit
EK5343 96 tests
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Human IL-17C ELISA Kit
GWB-SKR139 96 Tests Ask for price
Human IL-17C ELISA Kit
LF-EK50908 1×96T
EUR 777.6
Human IL-17C ELISA Kit
MBS8244654-5x96Tests 5x96Tests
EUR 2755
Human IL-17C ELISA Kit
MBS8244654-96Tests 96Tests
EUR 620
Recombinant Human IL-17C Protein
RP02709 1000μg
EUR 1218.75
Rabbit anti-IL-17C Antibody
DL97401A-100ul 100 ul
EUR 299
Description: Interleukin-17C; IL-17C; Cytokine CX2
Rabbit anti-IL-17C Antibody
DL97401A-50ul 50 ul
EUR 209.3
Description: Interleukin-17C; IL-17C; Cytokine CX2
Rabbit anti-IL-17C Antibody
YLD4294-100ul 100 ul
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Description: Rabbit polyclonal antibody to IL-17C
Rabbit anti-IL-17C Antibody
YLD4294-50ul 50 ul
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Description: Rabbit polyclonal antibody to IL-17C
Nori Human IL-17C ELISA Kit
GR111017 96-well
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Human IL-17C Protein, His Tag
ILC-H52H7 100ug
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Description: Human IL-17C, His Tag (ILC-H52H7) is expressed from human 293 cells (HEK293). It contains AA His 19 - Val 197 (Accession # Q9P0M4-1).
Rat Interleukin 17C(IL-17C)ELISA Kit
QY-E10618 96T
EUR 433.2
IL-17C (Human) LumiAb ELISA Kit
MBS9510893-10x96StripWells 10x96-Strip-Wells
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IL-17C (Human) LumiAb ELISA Kit
MBS9510893-5x96StripWells 5x96-Strip-Wells
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IL-17C (Human) LumiAb ELISA Kit
MBS9510893-96StripWells 96-Strip-Wells
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Mouse Interleukin 17C(IL-17C)ELISA Kit
QY-E20840 96T
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IL-17C Antibody
C30727-100ul 100μl
EUR 217
Description: IL-17C Rabbit Polyclonal Antibody
IL-17C Antibody
C30727-50ul 50μl
EUR 143.5
Description: IL-17C Rabbit Polyclonal Antibody
Human IL-17C PicoKine ELISA Kit
EK0789 96 wells
EUR 510
Description: For quantitative detection of human IL-17C in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Human IL-17C PicoKine ELISA Kit
MBS177124-5x96StripWells 5x96-Strip-Wells
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Human IL-17C PicoKine ELISA Kit
MBS177124-96StripWells 96-Strip-Wells
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IL-17C (Human) Sandwich ELISA Kit
OK-0239 1Kit
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Description: IL-17C (Human) Colorimetric Sandwich ELISA Kit
IL-17C (Human) OmniKine ELISA Kit
MBS9501965-1x96Wells 1x96Wells
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IL-17C (Human) OmniKine ELISA Kit
MBS9501965-5x96Wells 5x96Wells
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Anti-IL-17c Reference Antibody (MOR106)
E24CHA261 100 μg
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Description: Available in various conjugation types.
Rat Monoclonal anti-mouse IL-17C
mAP-0566 50ug
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OKAG00139-96W - IL-17C ELISA Kit (Human)
OKAG00139-96W 1plate:12x8-WellMicrostrips
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IL-17C Polyclonal Antibody
ABP58915-003ml 0.03ml
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Description: A polyclonal antibody for detection of IL-17C from Human. This IL-17C antibody is for IHC-P, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human IL-17C protein at amino acid sequence of 81-130
IL-17C Polyclonal Antibody
ABP58915-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of IL-17C from Human. This IL-17C antibody is for IHC-P, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human IL-17C protein at amino acid sequence of 81-130
IL-17C Polyclonal Antibody
ABP58915-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of IL-17C from Human. This IL-17C antibody is for IHC-P, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human IL-17C protein at amino acid sequence of 81-130
IL-17C Polyclonal Antibody
46856-100ul 100ul
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IL-17C Polyclonal Antibody
46856-50ul 50ul
EUR 224.4
IL-17C Polyclonal Antibody
E046856 100μg/100μl
EUR 255
Description: Available in various conjugation types.
IL-17C Polyclonal Antibody
E11-2035978 100ug/100ul
EUR 225
Description: Available in various conjugation types.
IL-17C Polyclonal Antibody
BT-AP10292-100ul 100ul Ask for price
Description: The protein encoded by this gene is a T cell-derived cytokine that shares the sequence similarity with IL17. This cytokine was reported to stimulate the release of tumor necrosis factor alpha and interleukin 1 beta from a monocytic cell line. The expression of this cytokine was found to be restricted to activated T cells.
IL-17C Polyclonal Antibody
BT-AP10292-20ul 20ul Ask for price
Description: The protein encoded by this gene is a T cell-derived cytokine that shares the sequence similarity with IL17. This cytokine was reported to stimulate the release of tumor necrosis factor alpha and interleukin 1 beta from a monocytic cell line. The expression of this cytokine was found to be restricted to activated T cells.
IL-17C Polyclonal Antibody
BT-AP10292-50ul 50ul Ask for price
Description: The protein encoded by this gene is a T cell-derived cytokine that shares the sequence similarity with IL17. This cytokine was reported to stimulate the release of tumor necrosis factor alpha and interleukin 1 beta from a monocytic cell line. The expression of this cytokine was found to be restricted to activated T cells.
IL-17C Polyclonal Antibody
E44H07420 100ul
EUR 255
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody
IL-17C Polyclonal Antibody
MBS9246588-01mL 0.1mL
EUR 415
IL-17C Polyclonal Antibody
MBS9246588-5x01mL 5x0.1mL
EUR 1841
IL-17C Polyclonal Antibody
MBS8532565-01mg 0.1mg
EUR 305
IL-17C Polyclonal Antibody
MBS8532565-01mLAF405L 0.1mL(AF405L)
EUR 465
IL-17C Polyclonal Antibody
MBS8532565-01mLAF405S 0.1mL(AF405S)
EUR 465
IL-17C Polyclonal Antibody
MBS8532565-01mLAF610 0.1mL(AF610)
EUR 465
  • To confirm that these mutations have been certainly accountable for the acquired resistance, transfection experiments have been carried out to re-establish MIL-susceptibility. In LEM3323, susceptibility was restored upon expression of a LiMT wild-type gene, whereas the MIL-susceptibility of LEM5159 might be reversed after expression of the LiRos3 wild-type gene.

 

  • The aberrant expression profile of the LiMT protein might be restored upon rescue of the LiRos3 gene each within the LEM5159 medical isolate and a ΔLiRos3 pressure, displaying that expression of LdMT relies on LdRos3 expression. The current findings clearly corroborate the pivotal position of the LiMT/LiRos3 advanced in resistance in the direction of MIL.