Current treatment for visceral leishmaniasis (VL), compromised by drug resistance, toxicity, and extreme worth, requires for less complicated, safer, and low-cost medication. Artemisinin has been found to be an effectual drug varied in experimental fashions of leishmaniasis. Comparative genome and transcriptome analysis of in vitro-adapted artesunate-resistant and -sensitive wild-type Leishmania donovani parasites was carried out using next-generation sequencing and single-color DNA microarray know-how, respectively, to determine genes and interlinked pathways contributing to drug resistance. Full-genome sequence and, 237 insertion deletions, 616 copy amount variations (CNVs) (377 deletions and 239 duplications), and trisomy of chromosome 12 in Okay133AS-R parasites. Transcriptome analysis revealed differential expression of 208 genes (fold change ≥ 2) in Okay133AS-R parasites.
Purposeful categorization and analysis of modulated genes of interlinked pathways recognized plausible permutations in Okay133AS-R parasites, paying homage to (i) a dependency on lipid and amino acid metabolism for producing energy, (ii) diminished DNA and protein synthesis leading to parasites throughout the quiescence state, and (iii) vigorous drug efflux. The upregulated expression of cathepsin-L like protease, amastin-like ground protein, and amino acid transporter and downregulated expression of the gene encoding ABCG2, pteridine receptor, adenylatecyclase-type receptor, phosphoaceylglucosamine mutase, and positive hypothetical proteins are concordant with genomic alterations suggesting their potential place in drug resistance. The look at equipped an understanding of the molecular basis linked to artemisinin resistance in Leishmania parasites, which will be advantageous for safeguarding this drug for future use.
Polyvinyl alcohol-based electrospun matrix as a provide system for nanoemulsion containing chalcone in direction of Leishmania (Leishmania) amazonensis
Cutaneous leishmaniasis is a worldwide public nicely being draw back. Typical therapies, together with the extreme worth, have many hostile outcomes and circumstances of parasite’s resistance. Chalcones are secondary metabolites precursors throughout the flavonoid pathway and is perhaps obtained naturally, nevertheless with low yield from plant raw supplies. Thus, the utilization of synthetic chalcones has been a promising approach for the occasion of molecules with leishmanicidal train.
Thus, this work aimed to develop a managed launch system of two synthetic chalcone (trans-chalcones and three’-(trifluormethyl)-chalcone) using polyvinyl alcohol nanofibers (PVA) as scaffold. The affiliation of chalcones to the nanofibers was made by nanoemulsions (NE) thereof, i.e., a colloidal system on a nanometric scale, which allows compounds with reverse polarities to remain miscible and regular all by their manipulation. Chalcone nanoemulsions had been developed using the spontaneous emulsification method. The NE had been characterised referring to their particle measurement, polydispersion index (PDI), and zeta potential.
The outcomes confirmed NE with spherical kind, absolute values of zeta potential had been larger than 30 mV and homogeneous distribution pattern (PDI < 0.3). Dynamics mild scattering (DLS) analysis confirmed comparable hydrodynamic rays, i.e., 180 nm (trans-chalcone NE) and 178 nm (NE containing 3′-(trifluormethyl)-chalcone, together with presenting encapsulation effectivity values close to 100 %. Subsequently, the NE had been added to a polymeric reply of polyvinyl alcohol (PVA) and processed by the electrospinning method affording a PVA matrix (15 %, w/v) nanofiber containing the chalcones NE at 1 mg.mL-1. In a follow-up experiment, the pores and pores and skin permeation assay of the PVA matrix-chalcone NE was carried out in vitro using Franz kind diffusion cells and porcine ear as natural model of look at.

Capsid building of Leishmania RNA virus 1
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MBS494629-5x96Tests | MyBiosource | 5x96Tests | EUR 3180 |
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MBS494629-96Tests | MyBiosource | 96Tests | EUR 695 |
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PCR-VH100-PCRVH10048D | Bioingentech | PCR-VH100-48D | EUR 230 |
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PCR-VH100-PCRVH10096D | Bioingentech | PCR-VH100-96D | EUR 312 |
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Oneq-VH10-OneqVH100100D | Bioingentech | Oneq-VH100-100D | EUR 515 |
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Oneq-VH10-OneqVH100150D | Bioingentech | Oneq-VH100-150D | EUR 594 |
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Oneq-VH10-OneqVH10050D | Bioingentech | Oneq-VH100-50D | EUR 413 |
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PCR-VH100-48D | Bioingentech | 50T | EUR 543.6 |
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PCR-VH100-96D | Bioingentech | 100T | EUR 686.4 |
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RTq-VH100-100D | Bioingentech | 100T | EUR 860.4 |
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RTq-VH100-150D | Bioingentech | 150T | EUR 969.6 |
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RTq-VH100-50D | Bioingentech | 50T | EUR 717.6 |
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Oneq-VH100-100D | Bioingentech | 100T | EUR 1039.2 |
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Oneq-VH100-150D | Bioingentech | 150T | EUR 1177.2 |
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Oneq-VH100-50D | Bioingentech | 50T | EUR 861.6 |
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MBS1215897-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1050 |
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MBS1215897-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 630 |
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MBS1215897-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 805 |
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MBS1215897-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 735 |
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MBS1215897-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 940 |
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MBS1140804-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1055 |
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MBS1140804-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 635 |
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MBS1140804-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 805 |
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MBS1140804-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 740 |
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MBS1140804-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 945 |
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MBS1090044-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1055 |
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MBS1090044-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 635 |
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MBS1090044-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 805 |
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MBS1090044-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 740 |
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MBS1090044-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 945 |
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MBS505114-1mg | MyBiosource | 1mg | EUR 1265 |
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MBS505114-5x1mg | MyBiosource | 5x1mg | EUR 5400 |
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QM4007 | Vivantis | each | Ask for price |
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VAng-Wyb7444-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5652 |
Description: Leishmania Infantum Flap endonuclease 1, recombinant protein. |
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VAng-Wyb7444-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 4002 |
Description: Leishmania Infantum Flap endonuclease 1, recombinant protein. |
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VAng-Wyb7444-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2733.6 |
Description: Leishmania Infantum Flap endonuclease 1, recombinant protein. |
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VAng-Wyb7449-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 3754.8 |
Description: Leishmania Infantum Ecotin-like protein 1, recombinant protein. |
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VAng-Wyb7449-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2683.2 |
Description: Leishmania Infantum Ecotin-like protein 1, recombinant protein. |
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VAng-Wyb7449-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1858.8 |
Description: Leishmania Infantum Ecotin-like protein 1, recombinant protein. |
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VAng-Wyb7450-inquire | Creative Biolabs | inquire | Ask for price |
Description: Leishmania Infantum Thymine dioxygenase JBP1, partial, recombinant protein. |
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VAng-Wyb7451-inquire | Creative Biolabs | inquire | Ask for price |
Description: Leishmania Infantum Bifunctional helicase and thymine dioxygenase JBP2, partial, recombinant protein. |
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VAng-Wyb7455-inquire | Creative Biolabs | inquire | Ask for price |
Description: Leishmania Infantum Major vault protein, partial, recombinant protein. |
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VAng-Wyb7456-inquire | Creative Biolabs | inquire | Ask for price |
Description: Leishmania Infantum Bifunctional NAD (P) H-hydrate repair enzyme, partial, recombinant protein. |
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VAng-Wyb7457-inquire | Creative Biolabs | inquire | Ask for price |
Description: Leishmania Infantum tRNA (guanine (37)-N1)-methyltransferase, partial, recombinant protein. |
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VAng-Wyb7458-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5256 |
Description: Leishmania Infantum 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase, recombinant protein. |
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VAng-Wyb7458-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3723.6 |
Description: Leishmania Infantum 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase, recombinant protein. |
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VAng-Wyb7458-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2551.2 |
Description: Leishmania Infantum 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase, recombinant protein. |
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VAng-Wyb7482-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5619.6 |
Description: Leishmania Infantum S-adenosylmethionine synthase, recombinant protein. |
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VAng-Wyb7482-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3606 |
Description: Leishmania Infantum S-adenosylmethionine synthase, recombinant protein. |
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VAng-Wyb7482-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2716.8 |
Description: Leishmania Infantum S-adenosylmethionine synthase, recombinant protein. |
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VAng-Wyb7485-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4546.8 |
Description: Leishmania Infantum 40S ribosomal protein S6, recombinant protein. |
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VAng-Wyb7485-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3244.8 |
Description: Leishmania Infantum 40S ribosomal protein S6, recombinant protein. |
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VAng-Wyb7485-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2205.6 |
Description: Leishmania Infantum 40S ribosomal protein S6, recombinant protein. |
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VAng-Wyb7483-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 3294 |
Description: Leishmania Infantum 60S ribosomal protein L37, recombinant protein. |
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VAng-Wyb7483-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2353.2 |
Description: Leishmania Infantum 60S ribosomal protein L37, recombinant protein. |
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VAng-Wyb7483-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1627.2 |
Description: Leishmania Infantum 60S ribosomal protein L37, recombinant protein. |
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VAng-Wyb7484-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 3556.8 |
Description: Leishmania Infantum 40S ribosomal protein S25, recombinant protein. |
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VAng-Wyb7484-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2551.2 |
Description: Leishmania Infantum 40S ribosomal protein S25, recombinant protein. |
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VAng-Wyb7484-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1759.2 |
Description: Leishmania Infantum 40S ribosomal protein S25, recombinant protein. |
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VAng-Wyb7459-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5718 |
Description: Leishmania Infantum Probable eukaryotic initiation factor 4A, recombinant protein. |
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VAng-Wyb7459-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 4035.6 |
Description: Leishmania Infantum Probable eukaryotic initiation factor 4A, recombinant protein. |
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VAng-Wyb7459-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2749.2 |
Description: Leishmania Infantum Probable eukaryotic initiation factor 4A, recombinant protein. |
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VAng-Wyb7460-inquire | Creative Biolabs | inquire | Ask for price |
Description: Leishmania Infantum Pescadillo homolog, partial, recombinant protein. |
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VAng-Wyb7461-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 3556.8 |
Description: Leishmania Infantum Anamorsin homolog, recombinant protein. |
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VAng-Wyb7461-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2551.2 |
Description: Leishmania Infantum Anamorsin homolog, recombinant protein. |
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VAng-Wyb7461-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1759.2 |
Description: Leishmania Infantum Anamorsin homolog, recombinant protein. |
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VAng-Wyb7462-inquire | Creative Biolabs | inquire | Ask for price |
Description: Leishmania Infantum Ubiquinone biosynthesis protein COQ4 homolog, mitochondrial, partial, recombinant protein. |
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VAng-Wyb7463-inquire | Creative Biolabs | inquire | Ask for price |
Description: Leishmania Infantum Ribosome biogenesis protein BOP1 homolog, partial, recombinant protein. |
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VAng-Wyb7464-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5751.6 |
Description: Leishmania Infantum ATPase ASNA1 homolog, recombinant protein. |
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VAng-Wyb7464-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 4068 |
Description: Leishmania Infantum ATPase ASNA1 homolog, recombinant protein. |
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VAng-Wyb7464-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2782.8 |
Description: Leishmania Infantum ATPase ASNA1 homolog, recombinant protein. |
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VAng-Wyb7465-inquire | Creative Biolabs | inquire | Ask for price |
Description: Leishmania Infantum Adenylosuccinate synthetase, partial, recombinant protein. |
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VAng-Wyb7466-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 3855.6 |
Description: Leishmania Infantum Ecotin-like protein 2, recombinant protein. |
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VAng-Wyb7466-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2749.2 |
Description: Leishmania Infantum Ecotin-like protein 2, recombinant protein. |
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VAng-Wyb7466-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1891.2 |
Description: Leishmania Infantum Ecotin-like protein 2, recombinant protein. |
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VAng-Wyb7467-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5520 |
Description: Leishmania Infantum Ecotin-like protein 3, recombinant protein. |
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VAng-Wyb7467-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3903.6 |
Description: Leishmania Infantum Ecotin-like protein 3, recombinant protein. |
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VAng-Wyb7467-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2667.6 |
Description: Leishmania Infantum Ecotin-like protein 3, recombinant protein. |
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VAng-Wyb7468-inquire | Creative Biolabs | inquire | Ask for price |
Description: Leishmania Infantum Probable citrate synthase, mitochondrial, partial, recombinant protein. |
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VAng-Wyb7469-inquire | Creative Biolabs | inquire | Ask for price |
Description: Leishmania Infantum Lipoyl synthase, mitochondrial, recombinant protein. |
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VAng-Wyb7470-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4514.4 |
Description: Leishmania Infantum Alpha-tubulin N-acetyltransferase, recombinant protein. |
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VAng-Wyb7470-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3228 |
Description: Leishmania Infantum Alpha-tubulin N-acetyltransferase, recombinant protein. |
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VAng-Wyb7470-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2188.8 |
Description: Leishmania Infantum Alpha-tubulin N-acetyltransferase, recombinant protein. |
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VAng-Wyb7471-inquire | Creative Biolabs | inquire | Ask for price |
Description: Leishmania Infantum Structure-specific endonuclease subunit SLX1 homolog, partial, recombinant protein. |
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VAng-Wyb7472-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4365.6 |
Description: Leishmania Infantum Probable methylthioribulose-1-phosphate dehydratase, recombinant protein. |
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VAng-Wyb7472-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3112.8 |
Description: Leishmania Infantum Probable methylthioribulose-1-phosphate dehydratase, recombinant protein. |
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VAng-Wyb7472-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2122.8 |
Description: Leishmania Infantum Probable methylthioribulose-1-phosphate dehydratase, recombinant protein. |
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VAng-Wyb7473-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5025.6 |
Description: Leishmania Infantum Guanine nucleotide-binding protein subunit beta-like protein, recombinant protein. |
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VAng-Wyb7473-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3556.8 |
Description: Leishmania Infantum Guanine nucleotide-binding protein subunit beta-like protein, recombinant protein. |
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VAng-Wyb7473-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2436 |
Description: Leishmania Infantum Guanine nucleotide-binding protein subunit beta-like protein, recombinant protein. |
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VAng-Wyb7474-inquire | Creative Biolabs | inquire | Ask for price |
Description: Leishmania Infantum Elongation factor Ts, mitochondrial, recombinant protein. |
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VAng-Wyb7475-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 9843.6 |
Description: Leishmania Infantum Protein SEY1 homolog, partial, recombinant protein. |
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VAng-Wyb7476-inquire | Creative Biolabs | inquire | Ask for price |
Description: Leishmania Infantum Translation factor GUF1 homolog, mitochondrial, partial, recombinant protein. |
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VAng-Wyb7477-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4646.4 |
Description: Leishmania Infantum 40S ribosomal protein S3a-1, recombinant protein. |
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VAng-Wyb7477-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3310.8 |
Description: Leishmania Infantum 40S ribosomal protein S3a-1, recombinant protein. |
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VAng-Wyb7477-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2254.8 |
Description: Leishmania Infantum 40S ribosomal protein S3a-1, recombinant protein. |
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VAng-Wyb7478-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4646.4 |
Description: Leishmania Infantum 40S ribosomal protein S3a-2, recombinant protein. |
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VAng-Wyb7478-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3310.8 |
Description: Leishmania Infantum 40S ribosomal protein S3a-2, recombinant protein. |
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VAng-Wyb7478-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2254.8 |
Description: Leishmania Infantum 40S ribosomal protein S3a-2, recombinant protein. |
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VAng-Wyb7479-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5504.4 |
Description: Leishmania Infantum Methylthioribose-1-phosphate isomerase, recombinant protein. |
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VAng-Wyb7479-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3886.8 |
Description: Leishmania Infantum Methylthioribose-1-phosphate isomerase, recombinant protein. |
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VAng-Wyb7479-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2650.8 |
Description: Leishmania Infantum Methylthioribose-1-phosphate isomerase, recombinant protein. |
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VAng-Wyb7480-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4514.4 |
Description: Leishmania Infantum 40S ribosomal protein SA, recombinant protein. |
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VAng-Wyb7480-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3228 |
Description: Leishmania Infantum 40S ribosomal protein SA, recombinant protein. |
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VAng-Wyb7480-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2188.8 |
Description: Leishmania Infantum 40S ribosomal protein SA, recombinant protein. |
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VAng-Wyb7443-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 3408 |
Description: Leishmania Infantum S-adenosylmethionine decarboxylase proenzyme, recombinant protein. |
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VAng-Wyb7443-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2436 |
Description: Leishmania Infantum S-adenosylmethionine decarboxylase proenzyme, recombinant protein. |
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VAng-Wyb7443-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1677.6 |
Description: Leishmania Infantum S-adenosylmethionine decarboxylase proenzyme, recombinant protein. |
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VAng-Wyb7452-100g | Creative Biolabs | 100 µg | EUR 1314 |
Description: Leishmania Infantum kinesin-like protein, recombinant protein. |
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VAng-Wyb7452-1mg | Creative Biolabs | 1 mg | EUR 3096 |
Description: Leishmania Infantum kinesin-like protein, recombinant protein. |
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VAng-Wyb7452-500g | Creative Biolabs | 500 µg | EUR 2205.6 |
Description: Leishmania Infantum kinesin-like protein, recombinant protein. |
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VAng-Wyb7481-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5108.4 |
Description: Leishmania Infantum 60S acidic ribosomal protein P0, recombinant protein. |
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VAng-Wyb7481-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3606 |
Description: Leishmania Infantum 60S acidic ribosomal protein P0, recombinant protein. |
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VAng-Wyb7481-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2469.6 |
Description: Leishmania Infantum 60S acidic ribosomal protein P0, recombinant protein. |
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MBS1001330-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1040 |
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MBS1001330-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 620 |
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MBS1001330-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 795 |
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MBS1001330-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 720 |
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MBS1001330-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 930 |
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VAng-Wyb7448-inquire | Creative Biolabs | inquire | Ask for price |
Description: Leishmania Infantum Heat shock protein 83-1, partial, recombinant protein. |
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VAng-Wyb7453-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 3342 |
Description: Leishmania Infantum Kinetoplastid membrane protein 11B, recombinant protein. |