Leishmania parasites trigger a broad spectrum of scientific illness. Right here we report the sequencing of the genomes of two species of Leishmania: Leishmania infantum and Leishmania braziliensis. The comparability of those sequences with the revealed genome of Leishmania main reveals marked conservation of synteny and identifies solely roughly 200 genes with a differential distribution between the three species.
L. braziliensis, opposite to Leishmania species examined to this point, possesses elements of a putative RNA-mediated interference pathway, telomere-associated transposable components and spliced leader-associated SLACS retrotransposons.
We present that pseudogene formation and gene loss are the principal forces shaping the totally different genomes. Genes which can be differentially distributed between the species encode proteins implicated in host-pathogen interactions and parasite survival within the macrophage.

Genomic and proteomic expression evaluation of Leishmania promastigote and amastigote life phases: the Leishmania genome is constitutively expressed.
- Leishmania are protozoan parasites that trigger a large spectrum of scientific ailments in people and are a serious public well being threat in a number of nations. Leishmania life cycle consists of an extracellular flagellated promastigote stage throughout the midgut of a sandfly vector, and a morphological distinct intracellular amastigote stage inside macrophages of a mammalian host.
Mouse Leukemia Inhibitory Factor (LIF) ELISA Kit |
DLR-LIF-Mu-96T |
DL Develop |
96T |
EUR 635 |
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Mouse Leukemia Inhibitory Factor (LIF) ELISA Kit |
RD-LIF-Mu-48Tests |
Reddot Biotech |
48 Tests |
EUR 489 |
Mouse Leukemia Inhibitory Factor (LIF) ELISA Kit |
RD-LIF-Mu-96Tests |
Reddot Biotech |
96 Tests |
EUR 677 |
Mouse Leukemia Inhibitory Factor (LIF) ELISA Kit |
RDR-LIF-Mu-48Tests |
Reddot Biotech |
48 Tests |
EUR 511 |
Mouse Leukemia Inhibitory Factor (LIF) ELISA Kit |
RDR-LIF-Mu-96Tests |
Reddot Biotech |
96 Tests |
EUR 709 |
anti-LIF |
YF-PA12952 |
Abfrontier |
50 ug |
EUR 363 |
Description: Mouse polyclonal to LIF |
Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit |
DLR-LIF-b-48T |
DL Develop |
48T |
EUR 547 |
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit |
DLR-LIF-b-96T |
DL Develop |
96T |
EUR 715 |
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Canine Leukemia Inhibitory Factor (LIF) ELISA Kit |
DLR-LIF-c-48T |
DL Develop |
48T |
EUR 527 |
Description: A sandwich quantitative ELISA assay kit for detection of Canine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Canine Leukemia Inhibitory Factor (LIF) ELISA Kit |
DLR-LIF-c-96T |
DL Develop |
96T |
EUR 688 |
Description: A sandwich quantitative ELISA assay kit for detection of Canine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Human Leukemia Inhibitory Factor (LIF) ELISA Kit |
DLR-LIF-Hu-48T |
DL Develop |
48T |
EUR 380 |
Description: A sandwich quantitative ELISA assay kit for detection of Human Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Human Leukemia Inhibitory Factor (LIF) ELISA Kit |
DLR-LIF-Hu-96T |
DL Develop |
96T |
EUR 485 |
Description: A sandwich quantitative ELISA assay kit for detection of Human Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit |
DLR-LIF-p-48T |
DL Develop |
48T |
EUR 547 |
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit |
DLR-LIF-p-96T |
DL Develop |
96T |
EUR 715 |
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Rat Leukemia Inhibitory Factor (LIF) ELISA Kit |
DLR-LIF-Ra-48T |
DL Develop |
48T |
EUR 508 |
Description: A sandwich quantitative ELISA assay kit for detection of Rat Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Rat Leukemia Inhibitory Factor (LIF) ELISA Kit |
DLR-LIF-Ra-96T |
DL Develop |
96T |
EUR 661 |
Description: A sandwich quantitative ELISA assay kit for detection of Rat Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit |
RD-LIF-b-48Tests |
Reddot Biotech |
48 Tests |
EUR 555 |
Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit |
RD-LIF-b-96Tests |
Reddot Biotech |
96 Tests |
EUR 771 |
Canine Leukemia Inhibitory Factor (LIF) ELISA Kit |
RD-LIF-c-48Tests |
Reddot Biotech |
48 Tests |
EUR 533 |
Canine Leukemia Inhibitory Factor (LIF) ELISA Kit |
RD-LIF-c-96Tests |
Reddot Biotech |
96 Tests |
EUR 740 |
Human Leukemia Inhibitory Factor (LIF) ELISA Kit |
RD-LIF-Hu-48Tests |
Reddot Biotech |
48 Tests |
EUR 367 |
Human Leukemia Inhibitory Factor (LIF) ELISA Kit |
RD-LIF-Hu-96Tests |
Reddot Biotech |
96 Tests |
EUR 502 |
Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit |
RD-LIF-p-48Tests |
Reddot Biotech |
48 Tests |
EUR 555 |
Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit |
RD-LIF-p-96Tests |
Reddot Biotech |
96 Tests |
EUR 771 |
Rat Leukemia Inhibitory Factor (LIF) ELISA Kit |
RD-LIF-Ra-48Tests |
Reddot Biotech |
48 Tests |
EUR 511 |
Rat Leukemia Inhibitory Factor (LIF) ELISA Kit |
RD-LIF-Ra-96Tests |
Reddot Biotech |
96 Tests |
EUR 709 |
Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit |
RDR-LIF-b-48Tests |
Reddot Biotech |
48 Tests |
EUR 580 |
Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit |
RDR-LIF-b-96Tests |
Reddot Biotech |
96 Tests |
EUR 807 |
Canine Leukemia Inhibitory Factor (LIF) ELISA Kit |
RDR-LIF-c-48Tests |
Reddot Biotech |
48 Tests |
EUR 557 |
Canine Leukemia Inhibitory Factor (LIF) ELISA Kit |
RDR-LIF-c-96Tests |
Reddot Biotech |
96 Tests |
EUR 774 |
Human Leukemia Inhibitory Factor (LIF) ELISA Kit |
RDR-LIF-Hu-48Tests |
Reddot Biotech |
48 Tests |
EUR 383 |
Human Leukemia Inhibitory Factor (LIF) ELISA Kit |
RDR-LIF-Hu-96Tests |
Reddot Biotech |
96 Tests |
EUR 525 |
Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit |
RDR-LIF-p-48Tests |
Reddot Biotech |
48 Tests |
EUR 580 |
Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit |
RDR-LIF-p-96Tests |
Reddot Biotech |
96 Tests |
EUR 807 |
Rat Leukemia Inhibitory Factor (LIF) ELISA Kit |
RDR-LIF-Ra-48Tests |
Reddot Biotech |
48 Tests |
EUR 534 |
Rat Leukemia Inhibitory Factor (LIF) ELISA Kit |
RDR-LIF-Ra-96Tests |
Reddot Biotech |
96 Tests |
EUR 742 |
anti- LIF antibody |
FNab09804 |
FN Test |
100µg |
EUR 548.75 |
Description: Antibody raised against LIF |
Anti-LIF Antibody |
A01400 |
BosterBio |
100ug/vial |
EUR 294 |
Anti-LIF Antibody |
PB9036 |
BosterBio |
100ug/vial |
EUR 334 |
Anti-LIF Antibody |
PA1562 |
BosterBio |
100ug/vial |
EUR 294 |
Anti-LIF antibody |
STJ96472 |
St John's Laboratory |
200 µl |
EUR 197 |
Description: Rabbit polyclonal to LIF. |
Anti-LIF antibody |
STJ29846 |
St John's Laboratory |
100 µl |
EUR 277 |
Description: The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
Rabbit Polyclonal antibody Anti-CRBN |
Anti-CRBN |
ImmunoStep |
50 µg |
EUR 349 |
Mouse LIF ELISA Kit |
EML0014 |
Abclonal |
96Tests |
EUR 521 |
Mouse LIF shRNA Plasmid |
20-abx971321 |
Abbexa |
|
|
Recombinant Mouse LIF Protein |
R01400-1 |
BosterBio |
100ug/vial |
EUR 294 |
Polyclonal Goat anti-GST α-form |
GST-ANTI-1 |
Detroit R&D |
50 uL |
EUR 280 |
Polyclonal Goat anti-GST μ-form |
GST-ANTI-2 |
Detroit R&D |
50 uL |
EUR 280 |
Polyclonal Goat anti-GST p-form |
GST-ANTI-3 |
Detroit R&D |
50 uL |
EUR 280 |
- This examine stories the usage of DNA oligonucleotide genome microarrays representing 8160 genes to investigate the mRNA expression profiles of L. main promastigotes and lesion derived amastigotes. Over 94% of the genes have been expressed in each life phases. Superior statistical evaluation recognized a surprisingly low diploma of differential mRNA expression: 1.4% of the overall genes in amastigotes and 1.5% in promastigotes.
- These microarray outcomes show that the L. main genome is actually constitutively expressed in each life phases and recommend that Leishmania is constitutively tailored for survival and replication in both the sandfly vector or macrophage host using an applicable set of genes for every vastly totally different atmosphere.
- Quantitative proteomics, utilizing the isotope coded affinity tag (ICAT) expertise and mass spectrometry, was used to establish L. infantum promastigote and axenic amastigote differentially expressed proteins.
- Of the 91 distinct proteins recognized, 8% have been differentially expressed within the amastigote stage, 20% have been differentially expressed within the promastigote stage, and the remaining 72% have been thought of constitutively expressed.
- The differential expression was validated by the identification of beforehand reported stage particular proteins and recognized a number of amastigote and promastigote novel stage particular proteins.
- To finish its life cycle, protozoan parasites of the genus Leishmania endure at the very least three main developmental transitions.
- Nevertheless, earlier efforts to establish genes exhibiting stage regulated adjustments in transcript abundance have yielded comparatively few. Right here we used expression profiling to evaluate adjustments in transcript abundance in three phases: replicating promastigotes and infective non-replicating metacyclics, which happen within the sand fly vector, and within the amastigote stage residing with macrophage phagolysosomes in mammals. Microarrays have been developed containing 11,484 PCR merchandise that included quite a lot of identified genes and 10,464 random 1 kb genomic DNA fragments.
IL-6, Interleukin-6, human |
RC212-17 |
BBI Biotech |
5ug |
EUR 104.38 |
IL-17, Interleukin-17 IL-17A, human |
RC212-28 |
BBI Biotech |
5ug |
EUR 104.38 |
Anti-IL-17 alpha antibody |
STJ93683 |
St John's Laboratory |
200 µl |
EUR 197 |
Description: Rabbit polyclonal to IL-17Ralpha. |
IL-17/IL-17A |
E21-021 |
EnoGene |
10ug |
EUR 343 |
IL-6, Interleukin-6, rat |
RC252-17 |
BBI Biotech |
2ug |
EUR 104.38 |
Recombinant Human IL-17 |
SJB09-03 |
Amyotop |
25µg/vial |
EUR 256 |
IL-6, Interleukin-6, murine (mouse) |
RC232-17 |
BBI Biotech |
2ug |
EUR 104.38 |
Interleukin 17 (IL-17) Antibody |
abx234224-100ug |
Abbexa |
100 ug |
EUR 509 |
Interleukin 17 (IL-17) Antibody |
abx234225-100ug |
Abbexa |
100 ug |
EUR 481 |
IL-6, Interleukin-6, monkey (rhesus macaque) |
RC222-17 |
BBI Biotech |
2ug |
EUR 104.38 |
IL-17 Antibody |
24784-100ul |
SAB |
100ul |
EUR 390 |
IL-17 Antibody |
24789-100ul |
SAB |
100ul |
EUR 390 |
Human Interleukin 17(IL-17)ELISA Kit |
GA-E0081HM-48T |
GenAsia Biotech |
48T |
EUR 289 |
Human Interleukin 17(IL-17)ELISA Kit |
GA-E0081HM-96T |
GenAsia Biotech |
96T |
EUR 466 |
Human Interleukin 17 (IL-17) CLIA Kit |
abx195860-96tests |
Abbexa |
96 tests |
EUR 825 |
Human Interleukin 17,IL-17 ELISA Kit |
201-12-0143 |
SunredBio |
96 tests |
EUR 440 |
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids. |
Human Interleukin 17,IL-17 ELISA Kit |
CN-03335H1 |
ChemNorm |
96T |
EUR 434 |
Human Interleukin 17,IL-17 ELISA Kit |
CN-03335H2 |
ChemNorm |
48T |
EUR 284 |
IL-17 Interleukin-17 Human Recombinant Protein |
PROTQ16552-1 |
BosterBio |
Regular: 25ug |
EUR 317 |
Description: Interleukin-17A Human Recombinant produced in E.Coli is a homodimeric, non-glycosylated polypeptide chain containing a total of 264 amino acids (2 chains of 132 aa) and having a molecular mass of 31kDa. ;The IL-17 is purified by proprietary chromatographic techniques. |
Recombinant Human IL-17 (IL-17A) Protein |
PROTQ16552-4 |
BosterBio |
25ug |
EUR 317 |
Description: The originally described IL-17 protein, now known as IL-17A, is a homodimer of two 136 amino acid chains, secreted by activated T-cells that act on stromal cells to induce production of proinflammatory and hematopoietic bioactive molecules. Today, IL-17 represents a family of structurally-related cytokines that share a highly conserved C-terminal region but differ from one another in their N-terminal regions and in their distinct biological roles. The six known members of this family, IL-17A through IL-17F, are secreted as homodimers. IL-17A exhibits cross-species bioactivity between human and murine cells. Recombinant human IL-17A is a 31.3 kDa disulfide-linked homodimer of two 137 amino acid polypeptide chains. |
Rabbit Polyclonal antibody Anti-CRBN |
Anti-CRBN |
ImmunoStep |
50 µg |
EUR 349 |
human IL-17, His tag |
E410A10-100 |
EnoGene |
100μg |
EUR 960 |
IL-17 (Human) ELISA Kit |
E4628-100 |
Biovision |
|
EUR 729 |
Human IL-17 ELISA kit |
LF-EK50201 |
Abfrontier |
1×96T |
EUR 648 |
Human Interleukin 17 (IL-17) Detection Assay Kit |
6808 |
Chondrex |
1 kit |
EUR 483.55 |
Description: Human Interleukin 17 (IL-17) Detection Assay Kit |
CLIA kit for Human IL-17 (Interleukin 17) |
E-CL-H0104 |
Elabscience Biotech |
1 plate of 96 wells |
EUR 584 |
Description: A sandwich CLIA kit for quantitative measurement of Human IL-17 (Interleukin 17) in samples from Serum, Plasma, Cell supernatant |
ELISA kit for Human IL-17 (Interleukin 17) |
E-EL-H0105 |
Elabscience Biotech |
1 plate of 96 wells |
EUR 377 |
Description: A sandwich ELISA kit for quantitative measurement of Human IL-17 (Interleukin 17) in samples from Serum, Plasma, Cell supernatant |
IL-17 Interleukin-17 Human Recombinant Protein, HEK |
PROTQ16552 |
BosterBio |
Regular: 10ug |
EUR 317 |
Description: IL-17 Human Recombinant produced in HEK cells is a glycosylated homodimer, having a molecular weight range of 30-35kDa due to glycosylation.;The IL-17 is purified by proprietary chromatographic techniques. |
17-Hydroxyprogesterone (17-OHP) ELISA Kit |
DLR-17-OHP-Ge-48T |
DL Develop |
48T |
EUR 469 |
Description: A competitive inhibition quantitative ELISA assay kit for detection of 17-Hydroxyprogesterone (17-OHP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
17-Hydroxyprogesterone (17-OHP) ELISA Kit |
DLR-17-OHP-Ge-96T |
DL Develop |
96T |
EUR 608 |
Description: A competitive inhibition quantitative ELISA assay kit for detection of 17-Hydroxyprogesterone (17-OHP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Polyclonal IL-17 Antibody |
APR06508G |
Leading Biology |
0.1 mg |
EUR 659 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human IL-17 . This antibody is tested and proven to work in the following applications: |
Polyclonal IL-17 Antibody |
APR06512G |
Leading Biology |
0.1 mg |
EUR 659 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human IL-17 . This antibody is tested and proven to work in the following applications: |
General 17-Hydroxyprogesterone (17-OHP) ELISA Kit |
RD-17-OHP-Ge-48Tests |
Cusabio |
48 Tests |
EUR 467 |
General 17-Hydroxyprogesterone (17-OHP) ELISA Kit |
RD-17-OHP-Ge-96Tests |
Reddot Biotech |
96 Tests |
EUR 646 |
General 17-Hydroxyprogesterone (17-OHP) ELISA Kit |
RDR-17-OHP-Ge-48Tests |
Reddot Biotech |
48 Tests |
EUR 488 |
General 17-Hydroxyprogesterone (17-OHP) ELISA Kit |
RDR-17-OHP-Ge-96Tests |
Reddot Biotech |
96 Tests |
EUR 676 |
Hamster IL-17(Interleukin 17) ELISA Kit |
EHA0007 |
FN Test |
96T |
EUR 567.6 |
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Hamster;Sensitivity: 18.75pg/ml |
Canine IL-17(Interleukin 17) ELISA Kit |
ECA0032 |
FN Test |
96T |
EUR 567.6 |
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Canine;Sensitivity: 18.75pg/ml |
Chicken IL-17(Interleukin 17) ELISA Kit |
ECH0038 |
FN Test |
96T |
EUR 567.6 |
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Chicken;Sensitivity: 9.375pg/ml |
- Arrays have been hybridized in triplicate and genes exhibiting two-fold or better adjustments in 2/three experiments have been scored as differentially expressed. Remarkably, solely about one p.c of the DNAs expression different by this standards, in both stage comparability. Northern blot evaluation confirmed the anticipated change in mRNA abundance for many of those (68%).
- This set of genes included most of those beforehand recognized within the literature as differentially regulated in addition to quite a lot of novel genes. Notably, Leishmania maxicircle transcripts confirmed sturdy up-regulation in metacyclic and amastigote parasites, most likely related to adjustments in parasite vitality metabolism. Nevertheless, present knowledge recommend that expression profiling utilizing shotgun DNA libraries considerably underestimates the extent of regulated transcripts.