Leishmania parasites trigger a broad spectrum of scientific illness. Right here we report the sequencing of the genomes of two species of Leishmania: Leishmania infantum and Leishmania braziliensis. The comparability of those sequences with the revealed genome of Leishmania main reveals marked conservation of synteny and identifies solely roughly 200 genes with a differential distribution between the three species.

L. braziliensis, opposite to Leishmania species examined to this point, possesses elements of a putative RNA-mediated interference pathway, telomere-associated transposable components and spliced leader-associated SLACS retrotransposons.

We present that pseudogene formation and gene loss are the principal forces shaping the totally different genomes. Genes which can be differentially distributed between the species encode proteins implicated in host-pathogen interactions and parasite survival within the macrophage.

 

 

Genomic and proteomic expression evaluation of Leishmania promastigote and amastigote life phases: the Leishmania genome is constitutively expressed.

 

• Leishmania are protozoan parasites that trigger a large spectrum of scientific ailments in people and are a serious public well being threat in a number of nations. Leishmania life cycle consists of an extracellular flagellated promastigote stage throughout the midgut of a sandfly vector, and a morphological distinct intracellular amastigote stage inside macrophages of a mammalian host.

Mouse Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-Mu-96T	DL Develop	96T	EUR 635
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Mouse Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-Mu-48Tests	Reddot Biotech	48 Tests	EUR 489

Mouse Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-Mu-96Tests	Reddot Biotech	96 Tests	EUR 677

Mouse Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-Mu-48Tests	Reddot Biotech	48 Tests	EUR 511

Mouse Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-Mu-96Tests	Reddot Biotech	96 Tests	EUR 709

anti-LIF
YF-PA12952	Abfrontier	50 ug	EUR 363
Description: Mouse polyclonal to LIF

Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-b-48T	DL Develop	48T	EUR 547
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids.

Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-b-96T	DL Develop	96T	EUR 715
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids.

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-c-48T	DL Develop	48T	EUR 527
Description: A sandwich quantitative ELISA assay kit for detection of Canine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-c-96T	DL Develop	96T	EUR 688
Description: A sandwich quantitative ELISA assay kit for detection of Canine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-Hu-48T	DL Develop	48T	EUR 380
Description: A sandwich quantitative ELISA assay kit for detection of Human Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-Hu-96T	DL Develop	96T	EUR 485
Description: A sandwich quantitative ELISA assay kit for detection of Human Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-p-48T	DL Develop	48T	EUR 547
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids.

Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-p-96T	DL Develop	96T	EUR 715
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids.

Rat Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-Ra-48T	DL Develop	48T	EUR 508
Description: A sandwich quantitative ELISA assay kit for detection of Rat Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-Ra-96T	DL Develop	96T	EUR 661
Description: A sandwich quantitative ELISA assay kit for detection of Rat Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-b-48Tests	Reddot Biotech	48 Tests	EUR 555

Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-b-96Tests	Reddot Biotech	96 Tests	EUR 771

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-c-48Tests	Reddot Biotech	48 Tests	EUR 533

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-c-96Tests	Reddot Biotech	96 Tests	EUR 740

Human Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-Hu-48Tests	Reddot Biotech	48 Tests	EUR 367

Human Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-Hu-96Tests	Reddot Biotech	96 Tests	EUR 502

Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-p-48Tests	Reddot Biotech	48 Tests	EUR 555

Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-p-96Tests	Reddot Biotech	96 Tests	EUR 771

Rat Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-Ra-48Tests	Reddot Biotech	48 Tests	EUR 511

Rat Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-Ra-96Tests	Reddot Biotech	96 Tests	EUR 709

Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-b-48Tests	Reddot Biotech	48 Tests	EUR 580

Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-b-96Tests	Reddot Biotech	96 Tests	EUR 807

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-c-48Tests	Reddot Biotech	48 Tests	EUR 557

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-c-96Tests	Reddot Biotech	96 Tests	EUR 774

Human Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-Hu-48Tests	Reddot Biotech	48 Tests	EUR 383

Human Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-Hu-96Tests	Reddot Biotech	96 Tests	EUR 525

Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-p-48Tests	Reddot Biotech	48 Tests	EUR 580

Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-p-96Tests	Reddot Biotech	96 Tests	EUR 807

Rat Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-Ra-48Tests	Reddot Biotech	48 Tests	EUR 534

Rat Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-Ra-96Tests	Reddot Biotech	96 Tests	EUR 742

anti- LIF antibody
FNab09804	FN Test	100µg	EUR 548.75
Description: Antibody raised against LIF

Anti-LIF Antibody
A01400	BosterBio	100ug/vial	EUR 294

Anti-LIF Antibody
PB9036	BosterBio	100ug/vial	EUR 334

anti- LIF antibody
LSMab09804	Lifescience Market	100 ug	EUR 386

Anti-LIF Antibody
PA1562	BosterBio	100ug/vial	EUR 294

Anti-LIF antibody
STJ73631	St John's Laboratory	100 µg	EUR 359

Anti-LIF antibody
STJ96472	St John's Laboratory	200 µl	EUR 197
Description: Rabbit polyclonal to LIF.

Anti-LIF antibody
STJ29846	St John's Laboratory	100 µl	EUR 277
Description: The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Rabbit Polyclonal antibody Anti-CRBN
Anti-CRBN	ImmunoStep	50 µg	EUR 349

LIF, Mouse
HY-P7084	MedChemExpress	50ug	EUR 533

LIF, mouse
MO16102	Neuromics	100 ug	EUR 675

Anti-LIF (aa28-39) antibody
STJ72919	St John's Laboratory	100 µg	EUR 359

Mouse LIF ELISA Kit
EML0014	Abclonal	96Tests	EUR 521

Mouse LIF shRNA Plasmid
20-abx971321	Abbexa	EUR 801.00 EUR 1121.00	150 µg 300 µg

Recombinant Mouse LIF Protein
R01400-1	BosterBio	100ug/vial	EUR 294

Polyclonal Goat anti-GST α-form
GST-ANTI-1	Detroit R&D	50 uL	EUR 280

Polyclonal Goat anti-GST μ-form
GST-ANTI-2	Detroit R&D	50 uL	EUR 280

Polyclonal Goat anti-GST p-form
GST-ANTI-3	Detroit R&D	50 uL	EUR 280

rHu LIF
AK9977-0005	Akron Biotech	5µg	Ask for price

rHu LIF
AK9977-0025	Akron Biotech	25µg	Ask for price

rHu LIF
AK9977-0100	Akron Biotech	100µg	Ask for price

rHu LIF
AK9977-1000	Akron Biotech	1mg	Ask for price

LIF, Human
HY-P7049	MedChemExpress	10ug	EUR 1600

LIF siRNA
20-abx922546	Abbexa	EUR 551.00 EUR 732.00	15 nmol 30 nmol

• This examine stories the usage of DNA oligonucleotide genome microarrays representing 8160 genes to investigate the mRNA expression profiles of L. main promastigotes and lesion derived amastigotes. Over 94% of the genes have been expressed in each life phases. Superior statistical evaluation recognized a surprisingly low diploma of differential mRNA expression: 1.4% of the overall genes in amastigotes and 1.5% in promastigotes.

 

• These microarray outcomes show that the L. main genome is actually constitutively expressed in each life phases and recommend that Leishmania is constitutively tailored for survival and replication in both the sandfly vector or macrophage host using an applicable set of genes for every vastly totally different atmosphere.

 

• Quantitative proteomics, utilizing the isotope coded affinity tag (ICAT) expertise and mass spectrometry, was used to establish L. infantum promastigote and axenic amastigote differentially expressed proteins.

 

• Of the 91 distinct proteins recognized, 8% have been differentially expressed within the amastigote stage, 20% have been differentially expressed within the promastigote stage, and the remaining 72% have been thought of constitutively expressed.

 

• The differential expression was validated by the identification of beforehand reported stage particular proteins and recognized a number of amastigote and promastigote novel stage particular proteins.

 

• To finish its life cycle, protozoan parasites of the genus Leishmania endure at the very least three main developmental transitions.

 

• Nevertheless, earlier efforts to establish genes exhibiting stage regulated adjustments in transcript abundance have yielded comparatively few. Right here we used expression profiling to evaluate adjustments in transcript abundance in three phases: replicating promastigotes and infective non-replicating metacyclics, which happen within the sand fly vector, and within the amastigote stage residing with macrophage phagolysosomes in mammals. Microarrays have been developed containing 11,484 PCR merchandise that included quite a lot of identified genes and 10,464 random 1 kb genomic DNA fragments.

Anti-IL-17 antibody
PAab04224	Lifescience Market	100 ug	EUR 386

IL-6, Interleukin-6, human
RC212-17	BBI Biotech	5ug	EUR 104.38

IL-17, Interleukin-17 IL-17A, human
RC212-28	BBI Biotech	5ug	EUR 104.38

IL-17, Human
HY-P7036	MedChemExpress	10ug	EUR 211

IL-17
E5-00007	EnoGene	1mg	EUR 960

IL-17
EF014060	Lifescience Market	96 Tests	EUR 689

IL-17
PR27113	Neuromics	5 ug	EUR 191

IL-17
MO15110	Neuromics	500 ug	EUR 910

Anti-IL-17 alpha antibody
STJ93683	St John's Laboratory	200 µl	EUR 197
Description: Rabbit polyclonal to IL-17Ralpha.

IL-17/IL-17A
E21-021	EnoGene	10ug	EUR 343

IL-6, Interleukin-6, rat
RC252-17	BBI Biotech	2ug	EUR 104.38

Recombinant Human IL-17
SJB09-03	Amyotop	25µg/vial	EUR 256

IL-6, Interleukin-6, murine (mouse)
RC232-17	BBI Biotech	2ug	EUR 104.38

Interleukin 17 (IL-17) Antibody
abx234224-100ug	Abbexa	100 ug	EUR 509

Interleukin 17 (IL-17) Antibody
abx234225-100ug	Abbexa	100 ug	EUR 481

Bovine Interleukin 17(IL-17)
QY-E60069	Qayee Biotechnology	96T	EUR 426

IL-6, Interleukin-6, monkey (rhesus macaque)
RC222-17	BBI Biotech	2ug	EUR 104.38

IL-17 Antibody
5176-100	Biovision		EUR 338

IL-17 Antibody
24784-100ul	SAB	100ul	EUR 390

IL-17 Antibody
24789-100ul	SAB	100ul	EUR 390

Human Interleukin 17(IL-17)ELISA Kit
GA-E0081HM-48T	GenAsia Biotech	48T	EUR 289

Human Interleukin 17(IL-17)ELISA Kit
GA-E0081HM-96T	GenAsia Biotech	96T	EUR 466

Human Interleukin 17 (IL-17) CLIA Kit
abx195860-96tests	Abbexa	96 tests	EUR 825

Human Interleukin 17,IL-17 ELISA Kit
201-12-0143	SunredBio	96 tests	EUR 440
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Interleukin 17,IL-17 ELISA Kit
CN-03335H1	ChemNorm	96T	EUR 434

Human Interleukin 17,IL-17 ELISA Kit
CN-03335H2	ChemNorm	48T	EUR 284

Human Interleukin 17(IL-17)ELISA Kit
QY-E04323	Qayee Biotechnology	96T	EUR 361

IL-17 Interleukin-17 Human Recombinant Protein
PROTQ16552-1	BosterBio	Regular: 25ug	EUR 317
Description: Interleukin-17A Human Recombinant produced in E.Coli is a homodimeric, non-glycosylated polypeptide chain containing a total of 264 amino acids (2 chains of 132 aa) and having a molecular mass of 31kDa.  ;The IL-17 is purified by proprietary chromatographic techniques.

Recombinant Human IL-17 (IL-17A) Protein
PROTQ16552-4	BosterBio	25ug	EUR 317
Description: The originally described IL-17 protein, now known as IL-17A, is a homodimer of two 136 amino acid chains, secreted by activated T-cells that act on stromal cells to induce production of proinflammatory and hematopoietic bioactive molecules.  Today, IL-17 represents a family of structurally-related cytokines that share a highly conserved C-terminal region but differ from one another in their N-terminal regions and in their distinct biological roles.  The six known members of this family, IL-17A through IL-17F, are secreted as homodimers.  IL-17A exhibits cross-species bioactivity between human and murine cells.  Recombinant human IL-17A is a 31.3 kDa disulfide-linked homodimer of two 137 amino acid polypeptide chains.

Rabbit Polyclonal antibody Anti-CRBN
Anti-CRBN	ImmunoStep	50 µg	EUR 349

human IL-17, His tag
E410A10-100	EnoGene	100μg	EUR 960

IL-17 (Human) ELISA Kit
E4628-100	Biovision		EUR 729

IL-17 ELISA KIT|Human
EF006939	Lifescience Market	96 Tests	EUR 689

Human IL-17 ELISA kit
LF-EK50201	Abfrontier	1×96T	EUR 648

Human Interleukin 17 (IL-17) Detection Assay Kit
6808	Chondrex	1 kit	EUR 483.55
Description: Human Interleukin 17 (IL-17) Detection Assay Kit

CLIA kit for Human IL-17 (Interleukin 17)
E-CL-H0104	Elabscience Biotech	1 plate of 96 wells	EUR 584
Description: A sandwich CLIA kit for quantitative measurement of Human IL-17 (Interleukin 17) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Human IL-17 (Interleukin 17)
E-EL-H0105	Elabscience Biotech	1 plate of 96 wells	EUR 377
Description: A sandwich ELISA kit for quantitative measurement of Human IL-17 (Interleukin 17) in samples from Serum, Plasma, Cell supernatant

IL-17 Interleukin-17 Human Recombinant Protein, HEK
PROTQ16552	BosterBio	Regular: 10ug	EUR 317
Description: IL-17 Human Recombinant produced in HEK cells is a glycosylated homodimer, having a molecular weight range of 30-35kDa due to glycosylation.;The IL-17 is purified by proprietary chromatographic techniques.

17-Hydroxyprogesterone (17-OHP) ELISA Kit
DLR-17-OHP-Ge-48T	DL Develop	48T	EUR 469
Description: A competitive inhibition quantitative ELISA assay kit for detection of 17-Hydroxyprogesterone (17-OHP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

17-Hydroxyprogesterone (17-OHP) ELISA Kit
DLR-17-OHP-Ge-96T	DL Develop	96T	EUR 608
Description: A competitive inhibition quantitative ELISA assay kit for detection of 17-Hydroxyprogesterone (17-OHP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

rHu IL-17-A
AK8249-0005	Akron Biotech	5µg	Ask for price

rHu IL-17-A
AK8249-0025	Akron Biotech	25µg	Ask for price

rHu IL-17-A
AK8249-0100	Akron Biotech	100µg	Ask for price

rHu IL-17-A
AK8249-1000	Akron Biotech	1mg	Ask for price

rHu IL-17-E
AK8299-0005	Akron Biotech	5µg	Ask for price

rHu IL-17-E
AK8299-0025	Akron Biotech	25µg	Ask for price

rHu IL-17-E
AK8299-0100	Akron Biotech	100µg	Ask for price

rHu IL-17-E
AK8299-1000	Akron Biotech	1mg	Ask for price

rHu IL-17-F
AK8312-0005	Akron Biotech	5µg	Ask for price

rHu IL-17-F
AK8312-0025	Akron Biotech	25µg	Ask for price

rHu IL-17-F
AK8312-0100	Akron Biotech	100µg	Ask for price

rHu IL-17-F
AK8312-1000	Akron Biotech	1mg	Ask for price

Polyclonal IL-17 Antibody
APR06508G	Leading Biology	0.1 mg	EUR 659
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human IL-17 . This antibody is tested and proven to work in the following applications:

Polyclonal IL-17 Antibody
APR06512G	Leading Biology	0.1 mg	EUR 659
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human IL-17 . This antibody is tested and proven to work in the following applications:

General 17-Hydroxyprogesterone (17-OHP) ELISA Kit
RD-17-OHP-Ge-48Tests	Cusabio	48 Tests	EUR 467

General 17-Hydroxyprogesterone (17-OHP) ELISA Kit
RD-17-OHP-Ge-96Tests	Reddot Biotech	96 Tests	EUR 646

General 17-Hydroxyprogesterone (17-OHP) ELISA Kit
RDR-17-OHP-Ge-48Tests	Reddot Biotech	48 Tests	EUR 488

General 17-Hydroxyprogesterone (17-OHP) ELISA Kit
RDR-17-OHP-Ge-96Tests	Reddot Biotech	96 Tests	EUR 676

Chicken Interleukin-17, IL-17 ELISA Kit
ELA-E0063d	Lifescience Market	96 Tests	EUR 928

Porcine Interleukin-17, IL-17 ELISA Kit
ELA-E0063p	Lifescience Market	96 Tests	EUR 928

Rat Interleukin 17, IL- 17 ELISA Kit
ELA-E0063r	Lifescience Market	96 Tests	EUR 886

Rabbit Interleukin-17, IL-17 ELISA Kit
ELA-E0063Rb	Lifescience Market	96 Tests	EUR 928

Hamster IL-17(Interleukin 17) ELISA Kit
EHA0007	FN Test	96T	EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Hamster;Sensitivity: 18.75pg/ml

Canine IL-17(Interleukin 17) ELISA Kit
ECA0032	FN Test	96T	EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Canine;Sensitivity: 18.75pg/ml

Chicken IL-17(Interleukin 17) ELISA Kit
ECH0038	FN Test	96T	EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Chicken;Sensitivity: 9.375pg/ml

• Arrays have been hybridized in triplicate and genes exhibiting two-fold or better adjustments in 2/three experiments have been scored as differentially expressed. Remarkably, solely about one p.c of the DNAs expression different by this standards, in both stage comparability. Northern blot evaluation confirmed the anticipated change in mRNA abundance for many of those (68%).

 

• This set of genes included most of those beforehand recognized within the literature as differentially regulated in addition to quite a lot of novel genes. Notably, Leishmania maxicircle transcripts confirmed sturdy up-regulation in metacyclic and amastigote parasites, most likely related to adjustments in parasite vitality metabolism. Nevertheless, present knowledge recommend that expression profiling utilizing shotgun DNA libraries considerably underestimates the extent of regulated transcripts.